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J Endod：Piezo通道在超声刺激的牙齿干细胞中的作用研究

2017-05-26 lishiting MedSci原创

Piezo超声干细胞增殖信号
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Piezo1和Piezo2是机械感应膜离子通道。研究者推测：在牙齿细胞中，Piezo蛋白对超声相关的机械信号转导以及下游胞外信号调节激酶（MAPK）信号的激活都可能起到一定的作用。这篇研究分别向牙髓干细胞（DPSCs）和牙周膜干细胞（PDLSCs）施加低强度的脉冲超声（LIPUS）刺激，检测Piezo通道的表达和作用。

Piezo1和Piezo2是机械感应膜离子通道。研究者推测：在牙齿细胞中，Piezo蛋白对超声相关的机械信号转导以及下游胞外信号调节激酶（MAPK）信号的激活都可能起到一定的作用。这篇研究分别向牙髓干细胞（DPSCs）和牙周膜干细胞（PDLSCs）施加低强度的脉冲超声（LIPUS）刺激，检测Piezo通道的表达和作用。

细胞增殖情况通过溴脱氧尿苷掺入进行评估。Western blot检测增殖细胞核抗原以及转录因子c-fos和c-jun的蛋白表达变化。细胞施加LIPUS刺激后，酶联免疫吸附测定法和western blot检测MAPK活性。细胞施加Piezo离子通道阻断剂-钌红（RR）刺激，检测Piezo蛋白在LIPUS刺激的细胞增殖和MAPK信号中的功能和作用。

Western blot结果显示，在2种细胞中均存在Piezo1和Piezo2的表达。施加LIPUS刺激的24 h后，DPSCs中Piezo蛋白的表达水平显著上调，而在PDLSCs中未观察到Piezo的明显变化。施加RR刺激显著抑制了LIPUS诱导的DPSCs增殖，而非PDLSCs。在施加LIPUS刺激后的24 h内，DPSCs中胞外信号相关激酶（ERK）1/2 MAPK信号处于持续的激活状态，而在PDLSCs中，磷酸化的c-Jun氨基末端激酶和p38胞外信号调节激酶MAPK的表达上调明显。RR在2种牙齿细胞类型中均可以影响MAPK信号活性，并且对ERK1/2/MAPK磷酸化水平的作用最为突出；而RR在DPSCs内对LIPUS诱导的ERK1/2活性增强起到明显的抑制作用表明，LIPUS刺激DPSCs的增殖涉及Piezo所介导的ERK1/2/MAPK信号调控。

结论：这篇研究第1次展示出Piezo离子通道在牙齿干细胞中对LIPUS应答的信号转导的作用。

原始出处：

Gao Q， Cooper PR， et al. Role of Piezo Channels in Ultrasound-stimulated Dental Stem Cells. J Endod. 2017 May 17. pii： S0099-2399(17)30267-4. doi： 10.1016/j.joen.2017.02.022.

本文系梅斯医学（MedSci）原创编译整理，转载需授权！

评论区 (6)
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    2017-06-12 zhanglin3079

    #Piezo#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1789065, encodeId=a8621e890659f, content=<a href='/topic/show?id=4133142e16e' target=_blank style='color:#2F92EE;'>#Piezo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14271, encryptionId=4133142e16e, topicName=Piezo)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e6a1280, createdName=zhanglin3079, createdTime=Mon Jun 12 18:11:00 CST 2017, time=2017-06-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1430083, encodeId=5f0f14300833e, content=<a href='/topic/show?id=b58f6e691fc' target=_blank style='color:#2F92EE;'>#牙齿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67691, encryptionId=b58f6e691fc, topicName=牙齿)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Sun May 28 11:11:00 CST 2017, time=2017-05-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=203021, encodeId=6aec20302118, content=学习了，谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=140, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zSn0GqHrsfLGSUSR8zwnWWLT4FMl5XH8S58HuZslVkLsAPbXDXaliagK7TOxQfmngKibG1yrDonepCLDkcxGk8hS/0, createdBy=b8282062892, createdName=changjiu, createdTime=Sat May 27 05:06:12 CST 2017, time=2017-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=202998, encodeId=1118202998cb, content=学习了谢谢分享。, beContent=null, objectType=article, channel=null, level=null, likeNumber=134, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=00501987275, createdName=130****4638, createdTime=Sat May 27 01:09:48 CST 2017, time=2017-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=202963, encodeId=a2552029633a, content=学习了很有用, beContent=null, objectType=article, channel=null, level=null, likeNumber=119, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Sat May 27 00:07:14 CST 2017, time=2017-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=202910, encodeId=02ac20291093, content=学习了，谢谢作者分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=112, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3xJXLtMtt5B40XXuPjn9xn1rBhrM6fHLkX1hI86ZoWoPJkgDkZuJNKkBfBHOcRd1fAdezg7JlHNOokohdAp3WkI/0, createdBy=32c81964262, createdName=1e145228m78(暂无匿称), createdTime=Fri May 26 22:20:57 CST 2017, time=2017-05-26, status=1, ipAttribution=)]
    2017-05-28 zhouqu_8

    #牙齿#

    0

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    2017-05-27 changjiu

    学习了，谢谢

    0

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    2017-05-27 130****4638

    学习了谢谢分享。

    0

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    2017-05-27 飛歌

    学习了很有用

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1789065, encodeId=a8621e890659f, content=<a href='/topic/show?id=4133142e16e' target=_blank style='color:#2F92EE;'>#Piezo#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14271, encryptionId=4133142e16e, topicName=Piezo)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=e6a1280, createdName=zhanglin3079, createdTime=Mon Jun 12 18:11:00 CST 2017, time=2017-06-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1430083, encodeId=5f0f14300833e, content=<a href='/topic/show?id=b58f6e691fc' target=_blank style='color:#2F92EE;'>#牙齿#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=83, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67691, encryptionId=b58f6e691fc, topicName=牙齿)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Sun May 28 11:11:00 CST 2017, time=2017-05-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=203021, encodeId=6aec20302118, content=学习了，谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=140, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zSn0GqHrsfLGSUSR8zwnWWLT4FMl5XH8S58HuZslVkLsAPbXDXaliagK7TOxQfmngKibG1yrDonepCLDkcxGk8hS/0, createdBy=b8282062892, createdName=changjiu, createdTime=Sat May 27 05:06:12 CST 2017, time=2017-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=202998, encodeId=1118202998cb, content=学习了谢谢分享。, beContent=null, objectType=article, channel=null, level=null, likeNumber=134, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=00501987275, createdName=130****4638, createdTime=Sat May 27 01:09:48 CST 2017, time=2017-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=202963, encodeId=a2552029633a, content=学习了很有用, beContent=null, objectType=article, channel=null, level=null, likeNumber=119, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Sat May 27 00:07:14 CST 2017, time=2017-05-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=202910, encodeId=02ac20291093, content=学习了，谢谢作者分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=112, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3xJXLtMtt5B40XXuPjn9xn1rBhrM6fHLkX1hI86ZoWoPJkgDkZuJNKkBfBHOcRd1fAdezg7JlHNOokohdAp3WkI/0, createdBy=32c81964262, createdName=1e145228m78(暂无匿称), createdTime=Fri May 26 22:20:57 CST 2017, time=2017-05-26, status=1, ipAttribution=)]
    2017-05-26 1e145228m78(暂无匿称)

    学习了，谢谢作者分享!

    0