J Endod:根尖周炎治疗后牙齿根尖系统的变化:微生物、形态和组织病理结果

2020-06-26 lishiting MedSci原创

这篇研究的目的是为了评估根尖周炎治疗后牙齿根尖系统的微生物条件变化,并将其与CBCT、micro-CT和组织病理学观察结果相关联。

这篇研究的目的是为了评估根尖周炎治疗后牙齿根尖系统的微生物条件变化,并将其与CBCT、micro-CT和组织病理学观察结果相关联。

研究从36颗需要实施牙周炎手术并且已行牙髓治疗的牙齿获取根尖。手术前进行CBCT扫描。根尖样本通过micro-CT进行扫描,随后低温粉碎。粉末进行DNA提取,随后通过实时定量聚合酶链式反应检测总细菌, Streptococcus species, members of the phylum Actinobacteria和Enterococcus faecalis。微生物结果与CBCT、micro-CT和组织病理数据结合进行评估。也评估损伤大小与未充填根尖系统体积比例之间的联系。

结果显示,所有低温粉碎的样本总细菌量均呈现阳性。放线菌和链球菌分别出现在35和33个样本中,并且通常在菌落中占主导地位。肉芽肿中放线菌计数是囊肿的2.23倍。链球菌在小损伤病例中的出现率更高。粪肠球菌仅在7个样本中检出,并且在菌落中总是占主导地位。单变量分析显示,未充填根管体积与总细菌、链球菌和放线菌计数存在显著相关性,但并未在校正分析中得到确认。大损伤与存在较大的未充填根尖体积显著相关。

结论:细菌感染发生在所有根尖组织,并且放线菌和链球菌占主导地位且检出率更高。未充填根尖系统的体积与损伤大小存在显著相关性,与细菌计数可能相关。结果表明,在牙髓治疗过程中,需要彻底去感染并且充填感染牙齿的根尖区。

原始出处:

José F Siqueira Jr, Henrique S Antunes, et al. The Apical Root Canal System of Teeth With Posttreatment Apical Periodontitis: Correlating Microbiologic, Tomographic and Histopathologic Findings. J Endod., 2020 Jun 7;S0099-2399(20)30383-6. doi: 10.1016/j.joen.2020.05.020.

 

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    #牙齿#

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