J Periodontol:小颗粒骨置换结合明胶支架保存牙槽嵴

2018-10-05 MedSci MedSci原创

颗粒状无机牛骨基质(ABBM)广泛用于牙槽嵴保存(ARP)。本研究评估了常规(250-1,000μm)和小(50-100μm)颗粒的ABBM /明胶冷冻凝胶支架对ARP的潜力。

颗粒状无机牛骨基质(ABBM)广泛用于牙槽嵴保存(ARP)。本研究评估了常规(250-1,000μm)和小(50-100μm)颗粒的ABBM /明胶冷冻凝胶支架对ARP的潜力。

方法:ABBM用石膏环压缩,以模拟缺陷中的ABBM填充,或者将其纳入明胶冷冻凝胶支架中以分散ABBM。将浓缩的常规尺寸或小尺寸的ABBM(rABBM或sABBM)(±骨形态发生蛋白[BMP] -2)皮下植入大鼠中以评估生物相容性和成骨潜力。在大鼠的上颌嵴上手术形成实验性拔牙窝,实验组和对照组进行不同的处理,未填充(对照),填充rABBM /明胶或sABBM /明胶冷冻凝胶支架。通过微计算机断层扫描成像和组织学评估拔牙窝填充和尺寸变化。

结果:与rABBM相比,压缩sABBM显示出可接受的生物相容性,但显着降低颗粒间距离(IPD)和孔隙率(P <0.001),而rABBM /明胶和sABBM /明胶冷冻凝胶支架显示出相似的IPD??和孔隙率。成骨作用发生在在8周时,rABBM /明胶中,sABBM /明胶处理的拔牙窝,并且与对照部位相比牙槽嵴宽增加,牙槽嵴差异减小。 sABBM /明胶支架在第4周显着增加牙槽窝填充并减少牙槽嵴差异,在第8周增加嵴宽度,并且在第4周和第8周减少颊嵴高度吸收(所有组均P <0.05)。

即使在添加BMP-2之后,压缩的sABBM组也抑制了骨传导性。通过将sABBM分散在明胶冷冻凝胶支架中,与rABBM /明胶相比,sABBM /明胶促进牙槽窝填充,在减少颊嵴萎缩和提供相等的脊稳定性方面显示出更大的潜力。

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