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Lab Chip：新型氧化石墨烯/聚多巴胺纳米界面可有效分离外泌体

2017-12-03 MedSci MedSci原创

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外泌体是细胞衍生的纳米囊泡，近来已经被认为是用于许多细胞过程的新的介质以及用于非侵入性疾病诊断和监测治疗反应的潜在生物标志物。为了更好地阐明外泌体的生物学和临床价值，迫切需要能够有效分离和灵敏分析这些小分子囊泡的新型分析技术。在此基础上，我们开发了基于新型氧化石墨烯/聚多巴胺（GO/PDA）纳米界面的微流体外泌体分析平台。据我们所知，本研究首次报道了由GO和PDA的微流体逐层沉积实现的GO诱导的3

外泌体是细胞衍生的纳米囊泡，近来已经被认为是用于许多细胞过程的新的介质以及用于非侵入性疾病诊断和监测治疗反应的潜在生物标志物。为了更好地阐明外泌体的生物学和临床价值，迫切需要能够有效分离和灵敏分析这些小分子囊泡的新型分析技术。

在此基础上，我们开发了基于新型氧化石墨烯/聚多巴胺（GO/PDA）纳米界面的微流体外泌体分析平台。据我们所知，本研究首次报道了由GO和PDA的微流体逐层沉积实现的GO诱导的3D纳米多孔PDA表面涂层的形成。

结果表明，这种纳米结构的GO/PDA界面大大提高了外泌体免疫捕获的效率，同时有效地抑制了非特异性外泌体吸附。基于这种纳米界面，开发了超灵敏的外泌体ELISA测定法，其检测极限为50μL-1，具有4个对数级的动态范围，比现有方法要灵敏。在临床上的应用，我们通过直接从2-μL血浆量化检测外泌体而不用样品处理来适应该平台来区分卵巢癌患者与健康对照者。

因此，这个平台可为基于外泌体的无创诊断及精准治疗提供了一个有用的工具。

原始出处：

Peng Zhang, Mei He, et al., Ultrasensitive Microfluidic Analysis of Circulating Exosomes Using a Nanostructured Graphene Oxide/Polydopamine Coating†. Lab Chip. 2016 Aug 2; 16(16): 3033–3042. doi:  10.1039/c6lc00279j


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#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1706952, encodeId=522a1e06952f7, content=<a href='/topic/show?id=d2773359191' target=_blank style='color:#2F92EE;'>#化石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=33591, encryptionId=d2773359191, topicName=化石)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f4d531331396, createdName=ms7899726347904398, createdTime=Tue May 08 04:11:00 CST 2018, time=2018-05-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651533, encodeId=b82b1651533de, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, createdName=yangjxjc, createdTime=Mon Oct 29 03:11:00 CST 2018, time=2018-10-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266817, encodeId=5ef726681ef9, content=还是这一篇...., beContent=null, objectType=article, channel=null, level=null, likeNumber=81, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Mon Dec 04 22:42:49 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266574, encodeId=74a42665e476, content=学习了感谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=94, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160914/IMG57D82E721DB581755.jpg, createdBy=4fc81933929, createdName=明天会更好！, createdTime=Sun Dec 03 23:58:43 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266537, encodeId=68c926653ead, content=学习了.涨知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sun Dec 03 21:33:20 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266521, encodeId=29f726652103, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sun Dec 03 21:18:46 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266519, encodeId=08b72665196f, content=学习了.前沿., beContent=null, objectType=article, channel=null, level=null, likeNumber=89, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Sun Dec 03 21:15:07 CST 2017, time=2017-12-03, status=1, ipAttribution=)]
    2018-05-08 ms7899726347904398

    #化石#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1706952, encodeId=522a1e06952f7, content=<a href='/topic/show?id=d2773359191' target=_blank style='color:#2F92EE;'>#化石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=33591, encryptionId=d2773359191, topicName=化石)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f4d531331396, createdName=ms7899726347904398, createdTime=Tue May 08 04:11:00 CST 2018, time=2018-05-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651533, encodeId=b82b1651533de, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, createdName=yangjxjc, createdTime=Mon Oct 29 03:11:00 CST 2018, time=2018-10-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266817, encodeId=5ef726681ef9, content=还是这一篇...., beContent=null, objectType=article, channel=null, level=null, likeNumber=81, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Mon Dec 04 22:42:49 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266574, encodeId=74a42665e476, content=学习了感谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=94, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160914/IMG57D82E721DB581755.jpg, createdBy=4fc81933929, createdName=明天会更好！, createdTime=Sun Dec 03 23:58:43 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266537, encodeId=68c926653ead, content=学习了.涨知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sun Dec 03 21:33:20 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266521, encodeId=29f726652103, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sun Dec 03 21:18:46 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266519, encodeId=08b72665196f, content=学习了.前沿., beContent=null, objectType=article, channel=null, level=null, likeNumber=89, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Sun Dec 03 21:15:07 CST 2017, time=2017-12-03, status=1, ipAttribution=)]
    2018-10-29 yangjxjc

    #石墨烯#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1706952, encodeId=522a1e06952f7, content=<a href='/topic/show?id=d2773359191' target=_blank style='color:#2F92EE;'>#化石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=33591, encryptionId=d2773359191, topicName=化石)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f4d531331396, createdName=ms7899726347904398, createdTime=Tue May 08 04:11:00 CST 2018, time=2018-05-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651533, encodeId=b82b1651533de, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, createdName=yangjxjc, createdTime=Mon Oct 29 03:11:00 CST 2018, time=2018-10-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266817, encodeId=5ef726681ef9, content=还是这一篇...., beContent=null, objectType=article, channel=null, level=null, likeNumber=81, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Mon Dec 04 22:42:49 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266574, encodeId=74a42665e476, content=学习了感谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=94, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160914/IMG57D82E721DB581755.jpg, createdBy=4fc81933929, createdName=明天会更好！, createdTime=Sun Dec 03 23:58:43 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266537, encodeId=68c926653ead, content=学习了.涨知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sun Dec 03 21:33:20 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266521, encodeId=29f726652103, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sun Dec 03 21:18:46 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266519, encodeId=08b72665196f, content=学习了.前沿., beContent=null, objectType=article, channel=null, level=null, likeNumber=89, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Sun Dec 03 21:15:07 CST 2017, time=2017-12-03, status=1, ipAttribution=)]
    2017-12-04 hlwss

    还是这一篇....

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1706952, encodeId=522a1e06952f7, content=<a href='/topic/show?id=d2773359191' target=_blank style='color:#2F92EE;'>#化石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=33591, encryptionId=d2773359191, topicName=化石)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f4d531331396, createdName=ms7899726347904398, createdTime=Tue May 08 04:11:00 CST 2018, time=2018-05-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651533, encodeId=b82b1651533de, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, createdName=yangjxjc, createdTime=Mon Oct 29 03:11:00 CST 2018, time=2018-10-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266817, encodeId=5ef726681ef9, content=还是这一篇...., beContent=null, objectType=article, channel=null, level=null, likeNumber=81, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Mon Dec 04 22:42:49 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266574, encodeId=74a42665e476, content=学习了感谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=94, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160914/IMG57D82E721DB581755.jpg, createdBy=4fc81933929, createdName=明天会更好！, createdTime=Sun Dec 03 23:58:43 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266537, encodeId=68c926653ead, content=学习了.涨知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sun Dec 03 21:33:20 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266521, encodeId=29f726652103, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sun Dec 03 21:18:46 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266519, encodeId=08b72665196f, content=学习了.前沿., beContent=null, objectType=article, channel=null, level=null, likeNumber=89, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Sun Dec 03 21:15:07 CST 2017, time=2017-12-03, status=1, ipAttribution=)]
    2017-12-03 明天会更好！

    学习了感谢分享!

    0

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    2017-12-03 1ddf0692m34(暂无匿称)

    学习了.涨知识

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1706952, encodeId=522a1e06952f7, content=<a href='/topic/show?id=d2773359191' target=_blank style='color:#2F92EE;'>#化石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=74, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=33591, encryptionId=d2773359191, topicName=化石)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f4d531331396, createdName=ms7899726347904398, createdTime=Tue May 08 04:11:00 CST 2018, time=2018-05-08, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651533, encodeId=b82b1651533de, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, createdName=yangjxjc, createdTime=Mon Oct 29 03:11:00 CST 2018, time=2018-10-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266817, encodeId=5ef726681ef9, content=还是这一篇...., beContent=null, objectType=article, channel=null, level=null, likeNumber=81, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Mon Dec 04 22:42:49 CST 2017, time=2017-12-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266574, encodeId=74a42665e476, content=学习了感谢分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=94, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20160914/IMG57D82E721DB581755.jpg, createdBy=4fc81933929, createdName=明天会更好！, createdTime=Sun Dec 03 23:58:43 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266537, encodeId=68c926653ead, content=学习了.涨知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=96, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sun Dec 03 21:33:20 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266521, encodeId=29f726652103, content=学习.谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=101, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyhb9xDeHojCicsdRdyC3XXdGk2ib9yLypZuHN06WeW4HO8WN0GGrgEfvnTX8gsckof05GHOU5OM4Mvx/0, createdBy=9cee1982656, createdName=执着追梦, createdTime=Sun Dec 03 21:18:46 CST 2017, time=2017-12-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=266519, encodeId=08b72665196f, content=学习了.前沿., beContent=null, objectType=article, channel=null, level=null, likeNumber=89, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/11/12/31d6593f5afbe66c4588cf468169340d.jpg, createdBy=ece52195975, createdName=hlwss, createdTime=Sun Dec 03 21:15:07 CST 2017, time=2017-12-03, status=1, ipAttribution=)]
    2017-12-03 执着追梦

    学习.谢谢分享

    0

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    2017-12-03 hlwss

    学习了.前沿.

    0