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J Exp Clin Cancer Res：细胞周期蛋白G2抑制Wnt /β-连环蛋白信号传导，并通过Dapper1抑制胃癌细胞的生长和迁移

2018-12-22 MedSci MedSci原创

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胃癌是最常见的恶性肿瘤之一，既往研究显示细胞周期蛋白G2与多种类型肿瘤的发展有关，但其在胃肿瘤中的潜在机制尚不清楚。本研究旨在探讨细胞周期蛋白G2对胃癌中Wnt/β-catenin信号通路的作用及其机制。使用实时荧光定量PCR，免疫组化和计算机模拟分析确定胃癌中cyclin G2的表达。TCGA数据集用于评估细胞周期蛋白G2表达与胃癌预后情况之间的关联。使用MTS测定，集落形成测定，细胞周期测定，

胃癌是最常见的恶性肿瘤之一，既往研究显示细胞周期蛋白G2与多种类型肿瘤的发展有关，但其在胃肿瘤中的潜在机制尚不清楚。本研究旨在探讨细胞周期蛋白G2对胃癌中Wnt/β-catenin信号通路的作用及其机制。

使用实时荧光定量PCR，免疫组化和计算机模拟分析确定胃癌中cyclin G2的表达。TCGA数据集用于评估细胞周期蛋白G2表达与胃癌预后情况之间的关联。使用MTS测定，集落形成测定，细胞周期测定，伤口愈合测定和transwell测定评估异位和内源细胞周期蛋白G2对胃癌细胞增殖和迁移的影响。此外，使用异种移植模型和裸鼠转移模型来确定细胞周期蛋白G2对胃肿瘤生长和体内迁移的影响。使用TOPFlash荧光素酶报告基因测定法研究细胞周期蛋白G2表达对Wnt /β-连环蛋白信号传导的影响，并使用免疫印迹测定，酵母双杂交筛选，免疫沉淀和Duolink原位PLA研究所涉及的分子机制构建Ccng2-/-小鼠以进一步证实细胞周期蛋白G2对体内Wnt /β-连环蛋白信号传导的抑制作用。此外，使用GSK-3β抑制剂探讨Wnt/β-catenin信号通路在细胞周期蛋白G2抑制胃癌细胞增殖和迁移中的作用。

结果显示，胃癌组织中细胞周期蛋白G2水平降低，并且与肿瘤大小、迁移和分化状态差有关。此外，细胞周期蛋白G2的过表达在体外和体内均减弱肿瘤生长和转移。Dpr1被鉴定为细胞周期蛋白G2相互作用蛋白，其是细胞周期蛋白G2介导的β-连环蛋白表达抑制所必需的。机制上，细胞周期蛋白G2影响CKI的活性以磷酸化Dpr1，已经证明Dpr1是一种蛋白质，其在未磷酸化时充当Wnt/β-连环蛋白信号传导的抑制剂。此外，GSK-3β抑制剂消除了细胞周期蛋白G2诱导的细胞增殖和迁移的抑制。

综上所述，该研究表明细胞周期蛋白G2抑制Wnt/β-连环蛋白信号传导并通过Dapper1抑制胃癌细胞生长和迁移。

原始出处：

Jinlan Gao, Chenyang Zhao, et al., Cyclin G2 suppresses Wnt/β-catenin signaling and inhibits gastric cancer cell growth and migration through Dapper1. J Exp Clin Cancer Res. 2018; 37: 317.

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    2019-02-02 ms8888626721583891

    #迁移#

    0

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    2019-07-31 ms7899726347904398

    #β-连环蛋白#

    0

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    2019-11-12 feather89

    #PE#

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    2019-01-09 kafei

    学习了谢谢

    0

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    2018-12-24 yxch36

    #癌细胞#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1640958, encodeId=ca0b1640958cf, content=<a href='/topic/show?id=7c99939e3fd' target=_blank style='color:#2F92EE;'>#迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93973, encryptionId=7c99939e3fd, topicName=迁移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d37c22750496, createdName=ms8888626721583891, createdTime=Sat Feb 02 03:08:00 CST 2019, time=2019-02-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1956402, encodeId=f761195640260, content=<a href='/topic/show?id=eafb192048e' target=_blank style='color:#2F92EE;'>#β-连环蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=19204, encryptionId=eafb192048e, topicName=β-连环蛋白)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/2898B09F0DA89357D0D54A725E611AC7/100, createdBy=fdd32500040, createdName=ms7899726347904398, createdTime=Wed Jul 31 23:08:00 CST 2019, time=2019-07-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727786, encodeId=c3081e27786e6, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Tue Nov 12 07:08:00 CST 2019, time=2019-11-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=357665, encodeId=95f435e665f7, content=学习了谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTLbTp9nZtjwVrSZNxAXbAicnKQWnic2eBdJUQYhxO36XdkstSicr7FVF8p5ReH4omtmsbnLdvZLiaJJ1A/132, createdBy=8bff2327056, createdName=kafei, createdTime=Wed Jan 09 18:22:04 CST 2019, time=2019-01-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1252562, encodeId=5162125256255, content=<a href='/topic/show?id=3efee1519b2' target=_blank style='color:#2F92EE;'>#癌细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71519, encryptionId=3efee1519b2, topicName=癌细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4cea26, createdName=yxch36, createdTime=Mon Dec 24 04:08:00 CST 2018, time=2018-12-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1287518, encodeId=5e97128e51857, content=<a href='/topic/show?id=1c48e783278' target=_blank style='color:#2F92EE;'>#细胞周期#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77832, encryptionId=1c48e783278, topicName=细胞周期)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8994206, createdName=chendoc242, createdTime=Mon Dec 24 04:08:00 CST 2018, time=2018-12-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1598629, encodeId=709c15986297e, content=<a href='/topic/show?id=c4da18e2588' target=_blank style='color:#2F92EE;'>#WNT#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=120, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=18725, encryptionId=c4da18e2588, topicName=WNT)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eb3518632538, createdName=zhwj, createdTime=Mon Dec 24 04:08:00 CST 2018, time=2018-12-24, status=1, ipAttribution=)]
    2018-12-24 chendoc242

    #细胞周期#

    0

  7. [GetPortalCommentsPageByObjectIdResponse(id=1640958, encodeId=ca0b1640958cf, content=<a href='/topic/show?id=7c99939e3fd' target=_blank style='color:#2F92EE;'>#迁移#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=84, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93973, encryptionId=7c99939e3fd, topicName=迁移)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=d37c22750496, createdName=ms8888626721583891, createdTime=Sat Feb 02 03:08:00 CST 2019, time=2019-02-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1956402, encodeId=f761195640260, content=<a href='/topic/show?id=eafb192048e' target=_blank style='color:#2F92EE;'>#β-连环蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=87, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=19204, encryptionId=eafb192048e, topicName=β-连环蛋白)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdqq.qlogo.cn/qqapp/1103841572/2898B09F0DA89357D0D54A725E611AC7/100, createdBy=fdd32500040, createdName=ms7899726347904398, createdTime=Wed Jul 31 23:08:00 CST 2019, time=2019-07-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1727786, encodeId=c3081e27786e6, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=80, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Tue Nov 12 07:08:00 CST 2019, time=2019-11-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=357665, encodeId=95f435e665f7, content=学习了谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=113, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTLbTp9nZtjwVrSZNxAXbAicnKQWnic2eBdJUQYhxO36XdkstSicr7FVF8p5ReH4omtmsbnLdvZLiaJJ1A/132, createdBy=8bff2327056, createdName=kafei, createdTime=Wed Jan 09 18:22:04 CST 2019, time=2019-01-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1252562, encodeId=5162125256255, content=<a href='/topic/show?id=3efee1519b2' target=_blank style='color:#2F92EE;'>#癌细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=71519, encryptionId=3efee1519b2, topicName=癌细胞)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4cea26, createdName=yxch36, createdTime=Mon Dec 24 04:08:00 CST 2018, time=2018-12-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1287518, encodeId=5e97128e51857, content=<a href='/topic/show?id=1c48e783278' target=_blank style='color:#2F92EE;'>#细胞周期#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=92, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77832, encryptionId=1c48e783278, topicName=细胞周期)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8994206, createdName=chendoc242, createdTime=Mon Dec 24 04:08:00 CST 2018, time=2018-12-24, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1598629, encodeId=709c15986297e, content=<a href='/topic/show?id=c4da18e2588' target=_blank style='color:#2F92EE;'>#WNT#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=120, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=18725, encryptionId=c4da18e2588, topicName=WNT)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=eb3518632538, createdName=zhwj, createdTime=Mon Dec 24 04:08:00 CST 2018, time=2018-12-24, status=1, ipAttribution=)]
    2018-12-24 zhwj

    #WNT#

    0