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J Endod：分子靶向环状di-AMP合成酶的活性会抑制粪肠球菌生长和生物膜形成

2018-08-09 lishiting MedSci原创

DisA粪肠球菌
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粪肠球菌因其生物膜形成的能力以及不同的毒力因子作用，常与根管治疗反复失败等口腔疾病相关联。Cyclic di-AMP (c-di-AMP)是一个遍在第二信使，存在于多种重要的细胞生理过程，包括生物膜形成。ST056083为小分子物质，它是体外c-di-AMP合成酶DNA完整性扫描蛋白(DisA)的抑制剂。这篇研究的目的是为了评估ST056083对粪肠球菌DisA活性、菌斑生长和生物膜形成的影响。

粪肠球菌因其生物膜形成的能力以及不同的毒力因子作用，常与根管治疗反复失败等口腔疾病相关联。Cyclic di-AMP (c-di-AMP)是一个遍在第二信使，存在于多种重要的细胞生理过程，包括生物膜形成。ST056083为小分子物质，它是体外c-di-AMP合成酶DNA完整性扫描蛋白(DisA)的抑制剂。这篇研究的目的是为了评估ST056083对粪肠球菌DisA活性、菌斑生长和生物膜形成的影响。研究采用Amber评分评估蛋白和配体的亲和力，采用UCSF Chimera (Resource for Biocomputing， Visualization， and Informatics， University of California， San Francisco， San Francisco， CA)分析和观察其结合方式。采用coralyne分析评估ST056083对粪肠球菌DisA活性的影响。采用生物膜定量分析、扫描电子显微镜和3D激光共聚焦扫描电镜测定评估ST056083对粪肠球菌菌斑生物膜形成的影响。采用anthrone-sulfuric法检测ST056083对粪肠球菌表多糖合

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    2019-06-17 yyanpro@21cn.c

    #分子靶向#

    0

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    2018-08-11 psybestwish

    #肠球菌#

    0

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    2018-08-11 hb2012ye

    #粪肠球菌#

    0