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JBMR Plus：新型异位成像窗口可用于工程化骨组织的活体成像

2018-10-06 MedSci MedSci原创

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组织工程是再生医学的一个有前途的分支，但是由于对这些工程化植入物的体内修复过程的有限了解限制了其临床应用。通常用于研究小鼠骨修复的不同阶段的技术是破坏性的，因此这些技术并不是最佳的。 而当与允许光学接近体内植入物的成像窗口组合时，多光子-活体显微镜（MP-IVM）允许在更长的时间段内以高分辨率和高频率可视化（亚）细胞过程。在这项研究中，我们开发并验证了异位成像窗口，该窗口可以放置在植入小鼠的

组织工程是再生医学的一个有前途的分支，但是由于对这些工程化植入物的体内修复过程的有限了解限制了其临床应用。通常用于研究小鼠骨修复的不同阶段的技术是破坏性的，因此这些技术并不是最佳的。

而当与允许光学接近体内植入物的成像窗口组合时，多光子-活体显微镜（MP-IVM）允许在更长的时间段内以高分辨率和高频率可视化（亚）细胞过程。在这项研究中，我们开发并验证了异位成像窗口，该窗口可以放置在植入小鼠的组织工程构建体上。这种方法不会干扰在这些植入物中发生的骨再生的生物过程，基于组织学和微计算机断层扫描（μCT）的对照异位植入物的比较已证明此点。异位成像窗口允许在体内数天内跟踪单个细胞。此外，荧光报道分子的使用允许在这些构建体中观察血管生成和骨生成的发生。

总之，这种新的成像窗口将有助于进一步分析骨组织工程植入物中细胞过程的时空调节，并提供增强骨组织工程治疗潜力的有力工具。

原始出处：

Stiers PJ, van Gastel N, et al., An Ectopic Imaging Window for Intravital Imaging of Engineered Bone Tissue. JBMR Plus. 2018 Jan 31;2(2):92-102. doi: 10.1002/jbm4.10028. eCollection 2018 Mar.

评论区 (3)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=2076343, encodeId=448120e634328, content=<a href='/topic/show?id=940410181964' target=_blank style='color:#2F92EE;'>#骨组织#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101819, encryptionId=940410181964, topicName=骨组织)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c192448, createdName=diushouji, createdTime=Sun Dec 09 10:46:00 CST 2018, time=2018-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2029660, encodeId=09012029660a0, content=<a href='/topic/show?id=fedb14394f4' target=_blank style='color:#2F92EE;'>#Plus#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=86, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14394, encryptionId=fedb14394f4, topicName=Plus)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/L2dMhrxjvSWuPWKZZP1I83Rw21EWia7LVTYJibLQkb4oTibrYLwhnAXntTBWR3FruIzKfNMiaBwhYV0AKNmiaNxfialg/132, createdBy=6ea92500119, createdName=ms9608593228839890, createdTime=Mon Sep 02 18:46:00 CST 2019, time=2019-09-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=348243, encodeId=b21134824368, content=不错的文章值得推荐, beContent=null, objectType=article, channel=null, level=null, likeNumber=110, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Sat Oct 06 23:23:33 CST 2018, time=2018-10-06, status=1, ipAttribution=)]
    2018-12-09 diushouji

    #骨组织#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=2076343, encodeId=448120e634328, content=<a href='/topic/show?id=940410181964' target=_blank style='color:#2F92EE;'>#骨组织#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101819, encryptionId=940410181964, topicName=骨组织)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c192448, createdName=diushouji, createdTime=Sun Dec 09 10:46:00 CST 2018, time=2018-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2029660, encodeId=09012029660a0, content=<a href='/topic/show?id=fedb14394f4' target=_blank style='color:#2F92EE;'>#Plus#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=86, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14394, encryptionId=fedb14394f4, topicName=Plus)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/L2dMhrxjvSWuPWKZZP1I83Rw21EWia7LVTYJibLQkb4oTibrYLwhnAXntTBWR3FruIzKfNMiaBwhYV0AKNmiaNxfialg/132, createdBy=6ea92500119, createdName=ms9608593228839890, createdTime=Mon Sep 02 18:46:00 CST 2019, time=2019-09-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=348243, encodeId=b21134824368, content=不错的文章值得推荐, beContent=null, objectType=article, channel=null, level=null, likeNumber=110, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Sat Oct 06 23:23:33 CST 2018, time=2018-10-06, status=1, ipAttribution=)]
    2019-09-02 ms9608593228839890

    #Plus#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=2076343, encodeId=448120e634328, content=<a href='/topic/show?id=940410181964' target=_blank style='color:#2F92EE;'>#骨组织#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=85, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101819, encryptionId=940410181964, topicName=骨组织)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c192448, createdName=diushouji, createdTime=Sun Dec 09 10:46:00 CST 2018, time=2018-12-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2029660, encodeId=09012029660a0, content=<a href='/topic/show?id=fedb14394f4' target=_blank style='color:#2F92EE;'>#Plus#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=86, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14394, encryptionId=fedb14394f4, topicName=Plus)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/L2dMhrxjvSWuPWKZZP1I83Rw21EWia7LVTYJibLQkb4oTibrYLwhnAXntTBWR3FruIzKfNMiaBwhYV0AKNmiaNxfialg/132, createdBy=6ea92500119, createdName=ms9608593228839890, createdTime=Mon Sep 02 18:46:00 CST 2019, time=2019-09-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=348243, encodeId=b21134824368, content=不错的文章值得推荐, beContent=null, objectType=article, channel=null, level=null, likeNumber=110, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=04f22055142, createdName=131****2916, createdTime=Sat Oct 06 23:23:33 CST 2018, time=2018-10-06, status=1, ipAttribution=)]
    2018-10-06 131****2916

    不错的文章值得推荐

    0