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J Periodontol：共栖菌群对牙周免疫应答脂多糖的影响

2018-05-26 lishiting MedSci原创

共栖菌群牙周免疫应答
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共生菌群参与了成熟免疫系统的发育。然而，它是如何促进免疫反应拮抗牙周病原菌的还不清楚，包括对脂多糖（LPS）的反应。这篇研究的目的通过对无菌(GF)和无特异性病原菌小鼠中局部施以LPS后，检测其免疫反应的表达变化。

共生菌群参与了成熟免疫系统的发育。然而，它是如何促进免疫反应拮抗牙周病原菌的还不清楚，包括对脂多糖（LPS）的反应。这篇研究的目的通过对无菌(GF)和无特异性病原菌小鼠中局部施以LPS后，检测其免疫反应的表达变化。

研究将8周龄的GF和SPF小鼠分别随机分为4组：对照组(n = 4/group)和3个实验组(n = 6/group)。实验组小鼠接受了牙龈卟啉单胞菌LPS (10 μg/μL)在上颌龈沟内的局部注射。在应用LPS之前以及应用LPS之后的3， 24或72小时，收集样本进行相应的处理。通过免疫组织化学评估牙周组织内的中性粒白细胞、CD4+和CD8+的T细胞数量。通过实时PCR检测编码细胞因子、炎症趋化因子和转录因子的基因表达。

结果显示，与施加LPS前相比，SPF小鼠在应用LPS 3小时后牙周组织内的CD4+ T细胞数量明显增加(p < 0.05)，而不是GF小鼠。在应用LPS3小时后，SPF小鼠的肿瘤坏死因子-α (Tnf-α)和叉头盒蛋白p3 (Foxp3)的基因表达明显高于GF小鼠(p < 0.05)。另外，GF和SPF小鼠在24小时时中性粒白细胞数量均达到最高。

结论：暴露于LPS下的SPF小鼠其CD4+ T细胞数量以及在牙周组织中的Tnf-α和Foxp3基因表达明显高于暴露于LPS下的GF小鼠。

原始出处：

Fukuhara D， Irie K， et al. Impact of commensal flora on periodontal immune response to lipopolysaccharide. J Periodontol. 2018 May 22. doi： 10.1002/JPER.17-0567.

本文系梅斯医学（MedSci）原创编译整理，转载需授权！

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    2019-03-31 feather89

    #PE#

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    2018-05-28 clmlylxy

    #免疫应答#

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    2018-05-28 小刀医生

    #脂多糖#

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    2018-05-28 liuyong2982

    #牙周#

    0

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    2018-05-26 131****1460

    学习了受益匪浅

    0

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    2018-05-26 清风拂面

    谢谢分享学习

    0

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    2018-05-26 惠映实验室

    学习了.谢谢分享.

    0