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CLIN CHEM：用于测定溶酶体酸脂肪酶的特定底物

2018-04-04 MedSci MedSci原创

溶酶体酸脂肪酶
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溶酶体酸性脂肪酶（LAL）的缺乏会导致沃尔曼病和胆固醇酯贮积病。随着最近引入酶替代疗法来控制LAL缺乏，目前，需要一种可应用于干血斑（DBS）的LAL酶活性准确测定的方法出现。 研究人员制备并测试了棕榈酰基4-甲基琥珀酰基酯类似物的文库，以发现DBS中的LAL高活性和特异性底物。通过对LAL分析的优化使同时对LAL的LC-MS / MS和荧光测定成为可能。研究人员采用新方法对来自健康和LAL

溶酶体酸性脂肪酶（LAL）的缺乏会导致沃尔曼病和胆固醇酯贮积病。随着最近引入酶替代疗法来控制LAL缺乏，目前，需要一种可应用于干血斑（DBS）的LAL酶活性准确测定的方法出现。 研究人员制备并测试了棕榈酰基4-甲基琥珀酰基酯类似物的文库，以发现DBS中的LAL高活性和特异性底物。通过对LAL分析的优化使同时对LAL的LC-MS / MS和荧光测定成为可能。研究人员采用新方法对来自健康和LAL缺陷患者的DBS进行了测定。 结果发现，与先前报道的LAL测定相比，使用P-PMHC和重同位素标记的内标和优化的测定条件使LAL的比活性增加约2倍。使用UPLC-MS / MS或荧光分析平台，通过新的LAL测定法可将LAL缺陷患者与正常人区分开来。 研究表明，该新方法可以测量DBS中的LAL，与之前的方法相比，单一测量方法可以同时进行2种测定。 原始出处： Sophia Masi, Naveen Chennamaneni, Frantisek Turecek. et.al. Specific Substrate for the Assay of Lysosomal Acid Lipase. 本文系梅

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  1. [GetPortalCommentsPageByObjectIdResponse(id=1672108, encodeId=1e3816e2108ed, content=<a href='/topic/show?id=860584396ef' target=_blank style='color:#2F92EE;'>#脂肪酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=98, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=84396, encryptionId=860584396ef, topicName=脂肪酶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=1fae26881555, createdName=tidiq, createdTime=Mon Oct 01 01:56:00 CST 2018, time=2018-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1662265, encodeId=27501662265f3, content=<a href='/topic/show?id=6a426545472' target=_blank style='color:#2F92EE;'>#测定#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65454, encryptionId=6a426545472, topicName=测定)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Sun Feb 17 20:56:00 CST 2019, time=2019-02-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302675, encodeId=0ea83026e5ff, content=很好的学习材料.对临床和科研都有很大帮助作用.水滴石穿.不断积累., beContent=null, objectType=article, channel=null, level=null, likeNumber=105, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzUn81b5CF5ibVPib8jWI92iciaxmqGHlwruOnK4SVZykd4iahqo1hicklIibyZoxm55X7EM8BW9eX1h1hQ2/0, createdBy=8aa81937526, createdName=李东泽, createdTime=Wed Apr 04 11:15:42 CST 2018, time=2018-04-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302672, encodeId=b4303026e29b, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/09/07/a9772c74ffd6edc03a7f9af07b4747f6.jpg, createdBy=b3722138742, createdName=sunfeifeiyang, createdTime=Wed Apr 04 11:08:59 CST 2018, time=2018-04-04, status=1, ipAttribution=)]
    2018-10-01 tidiq

    #脂肪酶#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1672108, encodeId=1e3816e2108ed, content=<a href='/topic/show?id=860584396ef' target=_blank style='color:#2F92EE;'>#脂肪酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=98, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=84396, encryptionId=860584396ef, topicName=脂肪酶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=1fae26881555, createdName=tidiq, createdTime=Mon Oct 01 01:56:00 CST 2018, time=2018-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1662265, encodeId=27501662265f3, content=<a href='/topic/show?id=6a426545472' target=_blank style='color:#2F92EE;'>#测定#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65454, encryptionId=6a426545472, topicName=测定)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Sun Feb 17 20:56:00 CST 2019, time=2019-02-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302675, encodeId=0ea83026e5ff, content=很好的学习材料.对临床和科研都有很大帮助作用.水滴石穿.不断积累., beContent=null, objectType=article, channel=null, level=null, likeNumber=105, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzUn81b5CF5ibVPib8jWI92iciaxmqGHlwruOnK4SVZykd4iahqo1hicklIibyZoxm55X7EM8BW9eX1h1hQ2/0, createdBy=8aa81937526, createdName=李东泽, createdTime=Wed Apr 04 11:15:42 CST 2018, time=2018-04-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302672, encodeId=b4303026e29b, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/09/07/a9772c74ffd6edc03a7f9af07b4747f6.jpg, createdBy=b3722138742, createdName=sunfeifeiyang, createdTime=Wed Apr 04 11:08:59 CST 2018, time=2018-04-04, status=1, ipAttribution=)]
    2019-02-17 爆笑小医

    #测定#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1672108, encodeId=1e3816e2108ed, content=<a href='/topic/show?id=860584396ef' target=_blank style='color:#2F92EE;'>#脂肪酶#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=98, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=84396, encryptionId=860584396ef, topicName=脂肪酶)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=1fae26881555, createdName=tidiq, createdTime=Mon Oct 01 01:56:00 CST 2018, time=2018-10-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1662265, encodeId=27501662265f3, content=<a href='/topic/show?id=6a426545472' target=_blank style='color:#2F92EE;'>#测定#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=66, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65454, encryptionId=6a426545472, topicName=测定)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=395f25, createdName=爆笑小医, createdTime=Sun Feb 17 20:56:00 CST 2019, time=2019-02-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302675, encodeId=0ea83026e5ff, content=很好的学习材料.对临床和科研都有很大帮助作用.水滴石穿.不断积累., beContent=null, objectType=article, channel=null, level=null, likeNumber=105, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/jW482SpianMayicTRbRZ5RzUn81b5CF5ibVPib8jWI92iciaxmqGHlwruOnK4SVZykd4iahqo1hicklIibyZoxm55X7EM8BW9eX1h1hQ2/0, createdBy=8aa81937526, createdName=李东泽, createdTime=Wed Apr 04 11:15:42 CST 2018, time=2018-04-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=302672, encodeId=b4303026e29b, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=97, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/09/07/a9772c74ffd6edc03a7f9af07b4747f6.jpg, createdBy=b3722138742, createdName=sunfeifeiyang, createdTime=Wed Apr 04 11:08:59 CST 2018, time=2018-04-04, status=1, ipAttribution=)]
    2018-04-04 李东泽

    很好的学习材料.对临床和科研都有很大帮助作用.水滴石穿.不断积累.

    0

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    2018-04-04 sunfeifeiyang

    阅

    0