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Biotechnol Rep：植物来源的重组骨桥蛋白-Fc融合蛋白可增强骨生成

2019-03-10 不详 网络

关注

骨桥蛋白（OPN）在骨再生过程中起重要作用。既往研究表明，重组人OPN能够在本塞姆氏烟草叶中表达并诱导成骨相关基因。然而，用Ni亲和层析从植物蛋白中纯化OPN仍然不够有效。 为了提高植物中蛋白质表达和纯化的质量，我们构建了基于Fc的OPN形式。 将完整的OPN蛋白与人IgG1 Fc结构域融合。在这里，我们显示植物产生的OPN-Fc增加蛋白质表达水平并促进重组蛋白质的纯化。 总之，该研究结果表明，植

骨桥蛋白（OPN）在骨再生过程中起重要作用。既往研究表明，重组人OPN能够在本塞姆氏烟草叶中表达并诱导成骨相关基因。然而，用Ni亲和层析从植物蛋白中纯化OPN仍然不够有效。

为了提高植物中蛋白质表达和纯化的质量，我们构建了基于Fc的OPN形式。 将完整的OPN蛋白与人IgG1 Fc结构域融合。在这里，我们显示植物产生的OPN-Fc增加蛋白质表达水平并促进重组蛋白质的纯化。

总之，该研究结果表明，植物产生的OPN-Fc可以刺激成骨相关基因如DMP1，OSX和Wnt3a的表达以及hPDL细胞中的钙沉积。 这些发现表明植物产生的OPN-Fc在将来具有潜在的组织工程应用。

原始出处：

Rattanapisit K, Srifa S, et al., Plant-produced recombinant Osteopontin-Fc fusion protein enhanced osteogenesis. Biotechnol Rep (Amst). 2019 Feb 8;21:e00312. doi: 10.1016/j.btre.2019.e00312. eCollection 2019 Mar.

评论区 (6)
#插入话题
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    2019-04-04 meichuangyi

    #融合蛋白#

    0

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    2019-05-12 shock_melon

    #TEC#

    0

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    2019-10-05 sunylz

    #Bio#

    0

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    2020-01-02 xuyong535

    #植物#

    0

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    2019-10-06 cathymary

    #Biotech#

    0

  6. [GetPortalCommentsPageByObjectIdResponse(id=1987947, encodeId=8f88198e9473c, content=<a href='/topic/show?id=00d88886e5e' target=_blank style='color:#2F92EE;'>#融合蛋白#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=89, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88867, encryptionId=00d88886e5e, topicName=融合蛋白)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=08e4137, createdName=meichuangyi, createdTime=Thu Apr 04 10:49:00 CST 2019, time=2019-04-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1652330, encodeId=f6c9165233035, content=<a href='/topic/show?id=13d01e24509' target=_blank style='color:#2F92EE;'>#TEC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=75, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17245, encryptionId=13d01e24509, topicName=TEC)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=852f24292001, createdName=shock_melon, createdTime=Sun May 12 06:49:00 CST 2019, time=2019-05-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1918570, encodeId=5b5e19185e040, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=93, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Sat Oct 05 03:49:00 CST 2019, time=2019-10-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1745311, encodeId=af961e4531110, content=<a href='/topic/show?id=bdc262694bb' target=_blank style='color:#2F92EE;'>#植物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=79, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=62694, encryptionId=bdc262694bb, topicName=植物)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=6a5e35527958, createdName=xuyong535, createdTime=Thu Jan 02 13:49:00 CST 2020, time=2020-01-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1927707, encodeId=3bbc192e70723, content=<a href='/topic/show?id=a59234e3b4' target=_blank style='color:#2F92EE;'>#Biotech#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=78, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3473, encryptionId=a59234e3b4, topicName=Biotech)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=226a143, createdName=cathymary, createdTime=Sun Oct 06 03:49:00 CST 2019, time=2019-10-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1525497, encodeId=73a4152549e3c, content=<a href='/topic/show?id=08548885835' target=_blank style='color:#2F92EE;'>#融合#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=69, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=88858, encryptionId=08548885835, topicName=融合)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=08a911636812, createdName=licz0427, createdTime=Tue Mar 12 09:49:00 CST 2019, time=2019-03-12, status=1, ipAttribution=)]
    2019-03-12 licz0427

    #融合#

    0