J Clin Periodontol:不同的探诊深度是否在微生物方面表现出明显的差异?

2018-01-28 MedSci MedSci原创

对慢性牙周炎(ChP)患者临床探诊的浅,中,深位点的龈下微生物群进行全面研究。

对慢性牙周炎(ChP)患者临床探诊的浅,中,深位点的龈下微生物群进行全面研究。

龈下标本来自ChP(n = 3 /探诊深度类别:≤3,4-6和≥7mm)和牙周健康(PH)的受试者。个别样本实验16S rDNA高通量测序和使用Mothur和R包分析。实验包括9名ChP和7名PH患者,评估了101个样本。检测到13门,118属和211个OTU。Chloroflexi和Spirochaetes门的分类群与疾病的初始阶段相关。:苛求依赖杆菌fretibacterium,优杆菌Eubacterium[XI] [G-6 ],脱硫杆菌(Desulfobulbus),消化链球菌科(Peptostreptococcaceae)[XI] [G-1]和[G-3],Bacteroidetes拟杆菌属[G-3],Bacteroidaceae拟杆菌属[G-1],Filifactor alocis ,Fretibacterium fastidiosum,Johnsonella spHOT166,消化链球菌科(Peptostreptococcaceae)[XIII] [G-1] HOT113,牙龈卟啉单胞菌(Porphyromonas endodontalis)和Treponema sp密螺旋体属螺旋体,HOT258,这些与传统疾病不相关,随着牙周袋深度增加和/或ChP升高而增加;而链球菌,棒状杆菌和Bergeyella属则与PH有关(P <0.05)。

结论:ChP个体在浅和中/深层的微生物群之间存在明显的差异,但是在中度和深度之间没有观察到明显的差异。还观察到PH和ChP的浅位点之间的差异。微生物群包括已知的口腔微生物和新鉴定的牙周分类群,其中一些尚未培养。

原始出处:

Paula Juliana Pérez-Chaparro, et al. Do different probing depths exhibit striking differences in microbial profiles?Journal of Clinical Periodontology. 2018 January. doi:10.1111/jcpe.12811

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J Clin Periodontol:探诊深度是否可以代表细菌种属的存在差异?

这篇研究的目的是为了全面评估慢性牙周炎(ChP)患者龈下浅、中和深部位存在的细菌种属的详细特征。